3Q02
Crystal structure of plasminogen activator inhibitor-1 in a metastable active conformation.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-08-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.438, 67.172, 90.357 |
| Unit cell angles | 90.00, 98.50, 90.00 |
Refinement procedure
| Resolution | 24.943 - 2.300 |
| R-factor | 0.1954 |
| Rwork | 0.193 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dvm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.026 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 40531 | |
| <I/σ(I)> | 17.6 | 2.1 |
| Completeness [%] | 98.7 | 97.9 |
| Redundancy | 4.7 | 4.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 1.7 - 3.0 M NaCl, 5 mM Mes pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
