3PZW
Soybean lipoxygenase-1 - re-refinement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1994-02-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.908 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.900, 94.000, 49.900 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.400 |
| R-factor | 0.15005 |
| Rwork | 0.149 |
| R-free | 0.16866 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1f8n |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.724 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.037 | 0.343 |
| Number of reflections | 161029 | |
| <I/σ(I)> | 35.7 | |
| Completeness [%] | 96.0 | 91.6 |
| Redundancy | 6.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | PEG 3350, SODIUM ACETATE, PH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
