3PZF
1.75A resolution structure of Serpin-2 from Anopheles gambiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-06 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 63 |
| Unit cell lengths | 96.538, 96.538, 78.289 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.254 - 1.750 |
| R-factor | 0.1751 |
| Rwork | 0.173 |
| R-free | 0.20690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Serpin-2 model from lower resolution data that was subsequently used for molecular replacement against this higher resolution data |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.233 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.2.3) |
| Refinement software | PHENIX (dev_605) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.059 | 0.415 |
| Number of reflections | 41856 | |
| <I/σ(I)> | 35.946 | 2.32 |
| Completeness [%] | 96.5 | 78.7 |
| Redundancy | 11.1 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 2.8 M Sodium Acetate, pH 7.0, vapor diffusion, temperature 293K |
