3PZ6
The crystal structure of GlLeuRS-CP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.280, 72.094, 111.249 |
| Unit cell angles | 90.00, 95.06, 90.00 |
Refinement procedure
| Resolution | 29.849 - 2.600 |
| R-factor | 0.2178 |
| Rwork | 0.215 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wfe |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.041 |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.600 |
| Number of reflections | 56769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 2% glycerol, 0.1M Bis-Tris pH 5.8, 2.2M ammonium sulfate, hanging drop, vapor diffusion, temperature 293K, VAPOR DIFFUSION, HANGING DROP |
