3PYX
Crystals Structure of Aspartate beta-Semialdehyde Dehydrogenase complex with NADP and 2-aminoterephthalate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-04 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.099, 99.280, 64.540 |
| Unit cell angles | 90.00, 101.02, 90.00 |
Refinement procedure
| Resolution | 29.330 - 1.600 |
| R-factor | 0.1611 |
| Rwork | 0.160 |
| R-free | 0.18202 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2gz3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.416 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.330 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 90598 | |
| <I/σ(I)> | 17.5 | 3.5 |
| Completeness [%] | 92.7 | 66.9 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 20 % PEG 3350, 0.1 M MES, 0.1 M ammonium acetate, 10 mM DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
