3PXJ
Tandem Ig repeats of Dlar
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.957, 77.513, 81.726 |
| Unit cell angles | 90.00, 101.08, 90.00 |
Refinement procedure
| Resolution | 41.385 - 2.300 |
| R-factor | 0.2251 |
| Rwork | 0.223 |
| R-free | 0.26130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pxh |
| RMSD bond length | 0.032 |
| RMSD bond angle | 1.657 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.117 | 0.108 | 0.415 |
| Number of reflections | 39287 | ||
| <I/σ(I)> | 12.3 | ||
| Completeness [%] | 98.7 | 95.7 | 98.2 |
| Redundancy | 2.7 | 2.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 50 mM imidazole pH 7.0, 10 mM ammonium sulfate, 20% (w/v) PEG 2000 MME, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
