3PX7

Crystal Structure of covalent complex of topoisomerase 1A with substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-01
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	61.602, 91.759, 141.976
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 2.300
R-factor	0.23
Rwork	0.230
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	500.000
High resolution limit [Å]	2.300
Number of reflections	65028
<I/σ(I)>	36.8
Completeness [%]	94.2
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6	288	0.2 M ammonium sulfate, 20% PEG4K, pH 6, VAPOR DIFFUSION, temperature 288K