3PWA
Structure of C126A mutant of Plasmodium falciparum triosephosphate isomerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-15 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.541 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 51.010, 175.360, 54.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.760 - 2.040 |
| R-factor | 0.18798 |
| Rwork | 0.186 |
| R-free | 0.23397 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.908 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.760 | 2.140 |
| High resolution limit [Å] | 2.039 | 2.030 |
| Number of reflections | 29675 | |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 92.3 | 75.3 |
| Redundancy | 10 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 24% PEG, Sodium Acetate buffer(pH 5.5), 10mM Lithium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
