3PW9

Structural and functional Analysis of Arabidopsis thaliana thylakoid lumen protein AtTLP18.3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97622
Spacegroup name	P 21 21 21
Unit cell lengths	47.147, 49.762, 76.464
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.500 - 2.100
Rwork	0.189
R-free	0.24800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3pvh
RMSD bond angle	0.012
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.063	0.036	0.496
Number of reflections	10968
<I/σ(I)>	26.4	36.3	3.7
Completeness [%]	99.5	96.8	99.9
Redundancy	5.3	4.7	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	296	0.2M Sodium acetate trihydrate, 0.1M Sodium cacodylate (pH 6.5), 25-30% (w/v) Polyethylene glycol 4000 , VAPOR DIFFUSION, HANGING DROP, temperature 296K