3PVW
Bovine GRK2 in complex with Gbetagamma subunits and a selective kinase inhibitor (CMPD103A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2009-08-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 187.564, 74.227, 123.346 |
Unit cell angles | 90.00, 115.65, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.490 |
R-factor | 0.2393 |
Rwork | 0.237 |
R-free | 0.28490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1omw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.141 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC (5.5.0102) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.590 |
High resolution limit [Å] | 2.490 | 5.380 | 2.500 |
Number of reflections | 50352 | ||
<I/σ(I)> | 20.45 | ||
Completeness [%] | 94.2 | 99.7 | 100 |
Redundancy | 3.8 | 3.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 277 | 7% PEG3350, 250 mM NaCl, 100 mM MES pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 277K |