3PVO
Monoclinic form of Human C-Reactive Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979689 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.600, 143.200, 161.200 |
| Unit cell angles | 90.00, 89.70, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.22946 |
| Rwork | 0.227 |
| R-free | 0.28521 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lj7 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.697 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 86781 | |
| <I/σ(I)> | 6.7 | 1.99 |
| Completeness [%] | 95.0 | 92.6 |
| Redundancy | 1.96 | 1.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 289 | 12% PEG 4000, 0.1M Tris-HCl, 0.2M MgCl2, 0.05M LiCl, pH 8.0, VAPOR DIFFUSION, temperature 289K |
