3PVL
Structure of myosin VIIa MyTH4-FERM-SH3 in complex with the CEN1 of Sans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 98.864, 98.864, 242.278 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.22922 |
| Rwork | 0.228 |
| R-free | 0.25903 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.980 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.874 |
| High resolution limit [Å] | 2.800 | 2.801 |
| Rmerge | 0.060 | 0.060 |
| Number of reflections | 32761 | |
| <I/σ(I)> | 31 | 31 |
| Completeness [%] | 99.6 | 99.69 |
| Redundancy | 9.1 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.02M MgCl2, 0.1M HEPES, pH 7.5, 22% (w/v) polyacrylic acid sodium salt 5100, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
