3PV7
Crystal structure of NKp30 ligand B7-H6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.502 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.300, 70.600, 53.200 |
Unit cell angles | 90.00, 100.40, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
Rwork | 0.246 |
R-free | 0.28630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.749 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
Rmerge | 0.067 | 0.045 | 0.604 |
Number of reflections | 21807 | ||
<I/σ(I)> | 13.1 | ||
Completeness [%] | 99.9 | 99.1 | 99.6 |
Redundancy | 7.3 | 7.2 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 7 | 298 | PEG 3350, LiCl2, pH 7.0, hanging drop, temperature 298K |