3PV6
Crystal structure of NKp30 bound to its ligand B7-H6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.502 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.874, 74.868, 125.534 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.239 |
| R-free | 0.28870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.988 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 21751 | |
| <I/σ(I)> | 53 | 3.3 |
| Completeness [%] | 98.4 | 88.2 |
| Redundancy | 14 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 7 | 298 | PEG 3350, KF, pH 7.0, hanging drop, temperature 298K |
