3PUY
Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to AMP-PNP after crystal soaking of the pretranslocation state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-05-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03324 |
Spacegroup name | P 1 |
Unit cell lengths | 76.550, 96.283, 112.283 |
Unit cell angles | 86.91, 80.87, 74.32 |
Refinement procedure
Resolution | 20.000 - 3.100 |
R-factor | 0.21629 |
Rwork | 0.214 |
R-free | 0.25427 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r6g |
RMSD bond length | 0.006 |
RMSD bond angle | 1.011 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.061 | |
Number of reflections | 46613 | |
<I/σ(I)> | 1.3 | |
Completeness [%] | 64.9 | 23.9 |
Redundancy | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 32% PEG 400, 0.1 M HEPES, 40 mM magnesium chloride, 50 mM sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.5 |