3PUS

PHF2 Jumonji-NOG-Ni(II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Detector technology	CCD
Collection date	2010-02-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	65.353, 96.979, 66.194
Unit cell angles	90.00, 89.78, 90.00

Refinement procedure

Resolution	33.530 - 2.080
R-factor	0.1774
Rwork	0.175
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kv4
RMSD bond length	0.007
RMSD bond angle	0.975
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.530	2.150
High resolution limit [Å]	2.080	2.080
Rmerge	0.085	0.629
Number of reflections	48987
<I/σ(I)>	14.3	2.2
Completeness [%]	99.3	99.9
Redundancy	3.4	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6		18-30% polyethylene glycol 3350 and 100 mM NaCitrate, pH 5.2-6.0, VAPOR DIFFUSION