3PUQ
CEKDM7A from C.Elegans, complex with alpha-KG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.592, 78.138, 102.930 |
| Unit cell angles | 90.00, 92.07, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.250 |
| R-factor | 0.1893 |
| Rwork | 0.188 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.040 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 69782 |
| Completeness [%] | 99.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 18% PEG 10000, 0.1M BIS-TRIS, 0.2M SODIUM FORMATE, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
