3PUJ
Crystal structure of the MUNC18-1 and SYNTAXIN4 N-Peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.95369 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 124.505, 124.505, 232.976 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.254 - 3.313 |
R-factor | 0.2129 |
Rwork | 0.211 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1DN1 Chain A |
RMSD bond length | 0.002 |
RMSD bond angle | 0.559 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.430 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.125 | 0.796 |
Number of reflections | 26564 | |
<I/σ(I)> | 10.3 | 1.8 |
Completeness [%] | 94.8 | 96.8 |
Redundancy | 6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 60% Tascimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |