3PU4
Crystal Structure of a vesicular stomatitis virus nucleocapsid-polyU complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 293 |
| Collection date | 2010-04-30 |
| Detector | MAR 325 CCD |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 165.477, 235.065, 75.498 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.774 - 3.000 |
| R-factor | 0.2519 |
| Rwork | 0.251 |
| R-free | 0.28830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.392 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.066 | 0.311 |
| Number of reflections | 51903 | |
| Completeness [%] | 86.5 | 45.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 5.5 % PEG 3350, 200 mM sodium chloride, 100mM sodium acetate, pH 4.5, vapor diffusion, hanging drop, temperature 293.0K |
