3PU1
Crystal Structure of a vesicular stomatitis virus nucleocapsid-polyG complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 293 |
| Collection date | 2009-01-20 |
| Detector | MAR 325 CCD |
| Wavelength(s) | 0.98008 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 163.222, 233.189, 75.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.100 - 3.140 |
| R-factor | 0.261 |
| Rwork | 0.259 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.354 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 3.260 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Rmerge | 0.094 | |
| Number of reflections | 42182 | |
| Completeness [%] | 82.6 | 64.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | 5.5 % PEG 3350, 200 mM sodium chloride, 100mM sodium acetate, pH 4.5, vapor diffusion, hanging drop, temperature 293.0K |
