3PTJ
Structural and functional Analysis of Arabidopsis thaliana thylakoid lumen protein AtTLP18.3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97884 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.977, 49.839, 76.672 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.600 |
Rwork | 0.199 |
R-free | 0.26900 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.590 | 2.600 |
Rmerge | 0.046 | 0.049 | 0.055 |
Number of reflections | 11594 | ||
<I/σ(I)> | 30.8 | 22.9 | 38.4 |
Completeness [%] | 97.8 | 94.4 | 99.6 |
Redundancy | 9.9 | 9.4 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 296 | 0.2M Sodium acetate trihydrate, 0.1M Sodium cacodylate, 25-30% (w/v) Polyethylene glycol 4000, 2mM TCEP , pH 6.0, VAPOR DIFFUSION, temperature 296K |