3PT3
Crystal structure of the C-terminal lobe of the human UBR5 HECT domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-05 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.240, 39.070, 44.170 |
| Unit cell angles | 90.04, 84.59, 81.77 |
Refinement procedure
| Resolution | 23.110 - 1.970 |
| R-factor | 0.22209 |
| Rwork | 0.219 |
| R-free | 0.27619 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c4z |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.296 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.010 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 11665 | |
| Completeness [%] | 90.8 | 93.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 0.1M Bis-Tris, 0.2M NaCl, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
