3PST
Crystal structure of PUL and PFU(mutate) domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 1W2B |
| Synchrotron site | BSRF |
| Beamline | 1W2B |
| Temperature [K] | 100 |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 61 |
| Unit cell lengths | 101.562, 101.562, 72.015 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.533 - 1.999 |
| R-factor | 0.1904 |
| Rwork | 0.188 |
| R-free | 0.22590 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.902 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 87.955 |
| High resolution limit [Å] | 1.999 |
| Number of reflections | 28692 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 0.15M Potassium bromide,30% w/v PEG 2000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
