3PSH
Classification of a Haemophilus influenzae ABC transporter HI1470/71 through its cognate molybdate periplasmic binding protein MolA (MolA bound to Molybdate)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.54, 1.00 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 123.295, 123.295, 103.037 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.940 - 1.500 |
| R-factor | 0.2976 |
| Rwork | 0.295 |
| R-free | 0.31670 |
| Structure solution method | SAD |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.384 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 138477 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 24% PEG2000 MME, 0.1 M sodium acetate pH 4.5, 0.2 M ammonium sulfate, 12% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
