3PRY
Crystal structure of the middle domain of human HSP90-beta refined at 2.3 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 31 |
| Unit cell lengths | 73.710, 73.710, 149.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.190 - 2.280 |
| R-factor | 0.17417 |
| Rwork | 0.172 |
| R-free | 0.21716 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1HK7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.364 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.190 | 2.400 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.085 | 0.637 |
| Number of reflections | 41218 | |
| <I/σ(I)> | 9.1 | 2 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 25.5% PEG3350, 0.17 M ammonium sulfate, 15% glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
