3PRO
ALPHA-LYTIC PROTEASE COMPLEXED WITH C-TERMINAL TRUNCATED PRO REGION
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03-05 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.150, 101.000, 72.140 |
| Unit cell angles | 90.00, 109.55, 90.00 |
Refinement procedure
| Resolution | 67.400 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tal |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.854) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.400 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.086 | 0.208 |
| Total number of observations | 176771 * | |
| Number of reflections | 73741 * | |
| <I/σ(I)> | 16.9 | 3.6 |
| Completeness [%] | 96.5 | 95.5 |
| Redundancy | 3.46 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 7 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 10 | 1 | reservoir | lithium sulfate | 0.8 (M) | bufferA |
| 11 | 1 | reservoir | glycerol | 5 (%) | bufferA |
| 12 | 1 | drop | bufferA | 0.45 times | |
| 2 | 1 | drop | lithium sulfate | 0.34 (M) | |
| 3 | 1 | drop | glycerol | 2.3 (%) | |
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | drop | MES | 2.7 (mM) | |
| 6 | 1 | reservoir | citric acid | 15 (mM) | bufferA |
| 7 | 1 | reservoir | phosphoric acid | 30 (mM) | bufferA |
| 8 | 1 | reservoir | orthoboric acid | 26 (mM) | bufferA |
| 9 | 1 | reservoir | sodium hydroxide | 0.155 (M) | bufferA |
