3PRK

INHIBITION OF PROTEINASE K BY METHOXYSUCCINYL-ALA-ALA-PRO-ALA-CHLOROMETHYL KETONE. AN X-RAY STUDY AT 2.2-ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	68.300, 68.300, 108.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.200
R-factor	0.198
RMSD bond length	0.009
RMSD bond angle	0.017 *
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Total number of observations	53620 *
Number of reflections	11864 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	5 (%(w/v))
2	1	drop	Tris-HCl	50 (mM)
3	1	drop		1 (mM)
4	1	reservoir		0.75 (M)
5	1	reservoir	Tris-HCl	50 (mM)