3PRD

Structural analysis of protein folding by the Methanococcus jannaschii chaperone FKBP26

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-01
Spacegroup name	I 41 2 2
Unit cell lengths	137.950, 137.950, 114.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 3.300
R-factor	0.3153
Rwork	0.315
R-free	0.32790
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.056
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	3.300
Number of reflections	8151

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop		298	1.5 M (NH4)2SO4, pH 100 mM Tris, pH 8.0, hanging drop, temperature 298K