3PR1
Crystal structure of apo Thermotoga maritima ribosome biogenesis GTP-binding protein EngB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-06-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54180 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.717, 97.988, 54.847 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.403 - 2.300 |
| R-factor | 0.2177 |
| Rwork | 0.216 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pqc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.951 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.970 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.102 |
| Number of reflections | 8326 |
| <I/σ(I)> | 19.4 |
| Completeness [%] | 96.0 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.1M bis-tris pH 5.5, 2.0M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
