3PQK

Crystal Structure of the transcriptional repressor BigR from Xylella fastidiosa

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-09-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97954, 0.9795, 0.9797, 0.9282
Spacegroup name	P 1
Unit cell lengths	34.230, 34.230, 140.830
Unit cell angles	90.03, 90.00, 120.03

Refinement procedure

Resolution	18.490 - 2.090
R-factor	0.2111
Rwork	0.209
R-free	0.24220
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	BUSTER (2.9.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.220
High resolution limit [Å]	2.090	2.090
Number of reflections	30801
<I/σ(I)>	11.33	2.8
Completeness [%]	94.0	92.4
Redundancy	2.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1