3PQK
Crystal Structure of the transcriptional repressor BigR from Xylella fastidiosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97954, 0.9795, 0.9797, 0.9282 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.230, 34.230, 140.830 |
| Unit cell angles | 90.03, 90.00, 120.03 |
Refinement procedure
| Resolution | 18.490 - 2.090 |
| R-factor | 0.2111 |
| Rwork | 0.209 |
| R-free | 0.24220 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.9.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.220 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 30801 | |
| <I/σ(I)> | 11.33 | 2.8 |
| Completeness [%] | 94.0 | 92.4 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
