3PQD

Crystal structure of L-lactate dehydrogenase from Bacillus subtilis complexed with FBP and NAD+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 31
Unit cell lengths	171.041, 171.041, 96.272
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.445 - 2.376
R-factor	0.2155
Rwork	0.214
R-free	0.26850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.445
High resolution limit [Å]	2.376
Number of reflections	126217
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20% PEGmme 2K, 0.1M Na/K PO4,pH 5.5, 1mM NAD+, 1mM FBP , VAPOR DIFFUSION, HANGING DROP, temperature 293K