3PQC
Crystal structure of Thermotoga maritima ribosome biogenesis GTP-binding protein EngB (YsxC/YihA) in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-06-18 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54180 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.670, 69.714, 94.836 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.709 - 1.900 |
R-factor | 0.183 |
Rwork | 0.180 |
R-free | 0.23170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1svi |
RMSD bond length | 0.005 |
RMSD bond angle | 0.947 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.710 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.070 | 0.366 |
Number of reflections | 33459 | |
<I/σ(I)> | 0.082 | 3.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2M sodium acetate pH5.5, 30% PEG 4000, 0.2M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 289K |