3PQ1
Crystal structure of human mitochondrial poly(A) polymerase (PAPD1)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96863, 0.97888, 0.97926 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.680, 76.880, 87.630 |
| Unit cell angles | 90.00, 103.39, 90.00 |
Refinement procedure
| Resolution | 28.912 - 3.100 |
| R-factor | 0.2514 |
| Rwork | 0.247 |
| R-free | 0.32840 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.373 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.080 | 0.289 |
| Number of reflections | 30725 | |
| <I/σ(I)> | 8.9681 | 2.342 |
| Completeness [%] | 97.7 | 90.1 |
| Redundancy | 2.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | The precipitant solution contained 100 mM MES (pH 6.0), 6% (v/v) PEG4000, 35 mM NaCl, and 5 mM MgSO4. The protein was at 20 mg/mL concentration, and 0.5 mM MgATP was included as an additive., microbatch, temperature 293K |
