3PPN

Structures of the substrate-binding protein provide insights into the multiple compatible solutes binding specificities of Bacillus subtilis ABC transporter OpuC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9194
Spacegroup name	P 21 21 21
Unit cell lengths	89.275, 89.667, 90.387
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.980 - 2.300
R-factor	0.2179
Rwork	0.217
R-free	0.23910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sw2
RMSD bond length	0.016
RMSD bond angle	1.406
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	32670
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	32%(v/v) Jeffamine M-600, 0.1M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K