3PPE

Crystal structure of chicken VE-cadherin EC1-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-04-09
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.97885
Spacegroup name	P 43 21 2
Unit cell lengths	99.973, 99.973, 105.987
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.740 - 2.100
R-factor	0.18692
Rwork	0.184
R-free	0.24189
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a4e
RMSD bond length	0.017
RMSD bond angle	1.666
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	31991
Completeness [%]	100.0	100
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	18% (w/v) PEG 8,000, 200mM Calcium acetate, 100mM sodium cacodylate pH6.5., VAPOR DIFFUSION, HANGING DROP, temperature 293.15K