3PP5

High-resolution structure of the trimeric Scar/WAVE complex precursor Brk1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0722
Spacegroup name	P 63
Unit cell lengths	39.140, 39.140, 118.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.389 - 1.500
R-factor	0.1601
Rwork	0.159
R-free	0.18170
Structure solution method	SAD, MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.245
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX ((anomalous dataset))
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.540
High resolution limit [Å]	1.500	1.500
Number of reflections	16181
<I/σ(I)>	30.4	8.95
Completeness [%]	98.6	97.4
Redundancy	12.5	12.09

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	40% (v/v) MPD, 0.2 M CaCl2, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.3	293	40% (v/v) MPD, 0.2 M CaCl2, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K