3POP
The crystal structure of GilR, an oxidoreductase that catalyzes the terminal step of gilvocarcin biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.075, 115.829, 290.104 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.972 - 1.651 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.19770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ipi |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.242 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_501)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.240 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 268870 | |
| <I/σ(I)> | 16.1 | 2.1 |
| Completeness [%] | 98.7 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM Tris, 200 mM MgCl2, 20% PEG 8000, 5-15% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
