3PNT
Crystal Structure of the Streptococcus pyogenes NAD+ glycohydrolase SPN in complex with IFS, the Immunity Factor for SPN
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | NOIR-1 |
Wavelength(s) | 0.97892, 0.97938, 0.99510 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 198.817, 57.892, 89.699 |
Unit cell angles | 90.00, 107.20, 90.00 |
Refinement procedure
Resolution | 42.720 - 2.800 |
R-factor | 0.21166 |
Rwork | 0.208 |
R-free | 0.27811 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.193 |
Refinement software | REFMAC (5.5.0093) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.720 |
High resolution limit [Å] | 2.800 |
Number of reflections | 24309 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 8000, NaCl, Tris (8.0), vapor diffusion, hanging drop, temperature 298K |