3PND

FAD binding by ApbE protein from Salmonella enterica: a new class of FAD binding proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	75
Detector technology	IMAGE PLATE
Collection date	2008-12-11
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	65.103, 120.850, 212.058
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.600 - 2.750
R-factor	0.205
Rwork	0.202
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o18
RMSD bond length	0.005
RMSD bond angle	0.899
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.25)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.600	32.599	2.820
High resolution limit [Å]	2.750	12.300	2.750
Rmerge	0.093	0.033	0.400
Total number of observations		2009	13860
Number of reflections	44268
<I/σ(I)>	14.7	12	1.9
Completeness [%]	99.7	91.6	99.9
Redundancy	4.2	3.8	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	277	0.5M LiSO4, 18% polyethylene glycol (PEG) 4000 in 100 mM Tris-HCl, and 10-20 mg/ml as purified FAD bound ApbE, pH 8.5, VAPOR DIFFUSION, temperature 277K