3PMT

Crystal structure of the Tudor domain of human Tudor domain-containing protein 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-12
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	41.682, 41.682, 59.947
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.690 - 1.800
R-factor	0.1789
Rwork	0.177
R-free	0.20890
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.161
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (refmac_5.6.0081)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.840
High resolution limit [Å]	1.800	4.440	1.800
Rmerge	0.031	0.021	0.099
Number of reflections	5478
<I/σ(I)>	35.4
Completeness [%]	98.7	99	85.8
Redundancy	19.5	21	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1M Tris-HCl pH 8.5, 2.0M Ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 291K