3PMS
Recombinant peptide: N-glycanase F (PNGase F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.960, 90.560, 48.810 |
| Unit cell angles | 90.00, 112.30, 90.00 |
Refinement procedure
| Resolution | 34.020 - 1.570 |
| R-factor | 0.1524 |
| Rwork | 0.151 |
| R-free | 0.17780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pgs |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.821 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.410 |
| High resolution limit [Å] | 1.570 |
| Rmerge | 0.057 |
| Number of reflections | 41972 |
| Completeness [%] | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295.15 | 25% PEG 4000, 0.2M ammonium sulfate, 0.1M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.15K |
