3PM9
Crystal structure of a Putative dehydrogenase (RPA1076) from Rhodopseudomonas palustris CGA009 at 2.57 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-10 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97922,0.97894 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 146.085, 250.728, 251.711 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.357 - 2.570 |
| R-factor | 0.1948 |
| Rwork | 0.193 |
| R-free | 0.22660 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.299 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.357 | 49.357 | 2.660 |
| High resolution limit [Å] | 2.570 | 5.500 | 2.570 |
| Rmerge | 0.114 | 0.039 | 0.824 |
| Number of reflections | 144716 | 14800 | 14230 |
| <I/σ(I)> | 10.26 | 25.5 | 1.7 |
| Completeness [%] | 98.9 | 96 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.60M potassium dihydrogen phosphate, 25.00% Glycerol, 0.60M sodium dihydrogen phosphate, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
