3PM2

Crystal structure of a novel type of odorant binding protein from Anopheles gambiae belonging to the c+ class

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97625
Spacegroup name	P 41 21 2
Unit cell lengths	36.289, 36.289, 263.541
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.990 - 1.800
R-factor	0.1887
Rwork	0.187
R-free	0.22720
Structure solution method	remote SAD
RMSD bond length	0.011
RMSD bond angle	1.020
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.300	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.167	0.550
Number of reflections	17578
<I/σ(I)>	19.3	4
Completeness [%]	99.9	100
Redundancy	11.5	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	Protein 12 mg/ml, 30-35% PEG5000 MME, 0.1 M sodium acetate pH 5.7-6.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	6	298	Protein 12 mg/ml, 30-35% PEG5000 MME, 0.1 M sodium acetate pH 5.7-6.3, VAPOR DIFFUSION, SITTING DROP, temperature 298K