3PM1
Structure of QacR E90Q bound to Ethidium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 298 |
Collection date | 2005-04-23 |
Wavelength(s) | 1.0 |
Spacegroup name | P 62 |
Unit cell lengths | 104.148, 104.148, 98.564 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.090 - 2.800 |
R-factor | 0.229 |
Rwork | 0.229 |
R-free | 0.28000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3RB2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.100 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 2.980 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 13889 | |
Completeness [%] | 92.0 | 92 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | ammonium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |