3PLT
Crystal structure of Lsp1 from Saccharomyces cerevisiae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979700 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 273.160, 38.753, 75.509 |
Unit cell angles | 90.00, 99.35, 90.00 |
Refinement procedure
Resolution | 37.510 - 2.900 |
R-factor | 0.239 |
Rwork | 0.236 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.130 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.106 | 0.896 |
Number of reflections | 17725 | |
<I/σ(I)> | 18.75 | 1.91 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 6.8 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 11 | 293 | 0.2M NaCl, 20mM phenol, 20% PEG 8000, 0.1M CAPS, pH 11, VAPOR DIFFUSION, HANGING DROP, temperature 293K |