3PL0

Crystal structure of a bsmA homolog (Mpe_A2762) from Methylobium petroleophilum PM1 at 1.91 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9537,0.9796,0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	43.508, 63.203, 104.504
Unit cell angles	90.00, 97.80, 90.00

Refinement procedure

Resolution	28.923 - 1.910
R-factor	0.1715
Rwork	0.169
R-free	0.21090
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.512
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.923	28.923	1.980
High resolution limit [Å]	1.910	4.100	1.910
Rmerge	0.042	0.022	0.543
Number of reflections	43643	8095	8670
<I/σ(I)>	10.4	26.4	1.9
Completeness [%]	96.6	92.6	97.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.90M lithium chloride, 6.00% polyethylene glycol 6000, 0.1M HEPES pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K