3PKZ

Structural basis for catalytic activation of a serine recombinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	73
Detector technology	CCD
Collection date	2009-11-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 65
Unit cell lengths	128.208, 128.208, 182.015
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.970 - 1.800
R-factor	0.20886
Rwork	0.207
R-free	0.24645
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.936
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.860
High resolution limit [Å]	1.800	1.830
Rmerge		0.966
Number of reflections	140384
<I/σ(I)>		1.79
Completeness [%]	90.0
Redundancy		6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	1.8M Ammonium Sulfate, 100 mM HEPES pH 7.0, 20% Ethylene Glycol, 5% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 292K
1	VAPOR DIFFUSION, HANGING DROP	7	292	1.8M Ammonium Sulfate, 100 mM HEPES pH 7.0, 20% Ethylene Glycol, 5% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 292K