3PJI
Crystal structure of AgamOBP22a at 1.7 angstrom in the open status for ligand binding.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-06 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.263, 37.702, 43.639 |
| Unit cell angles | 90.00, 99.46, 90.00 |
Refinement procedure
| Resolution | 25.424 - 1.704 |
| R-factor | 0.1822 |
| Rwork | 0.179 |
| R-free | 0.20810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.932 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 11797 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.8 M Ammonium citrate tribasic, 3.5% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
