3PJ7
Crystal structure of far-red fluorescent protein Katushka crystallized at pH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 41 |
| Unit cell lengths | 161.353, 161.353, 74.486 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.990 - 1.850 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.139 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 3.880 | 1.800 |
| Rmerge | 0.096 | 0.066 | |
| Number of reflections | 88163 | ||
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 5 | 5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 24% w/v PEG4000, 0.16 M MgCl2, 0.08 M TRIS hydrochloride pH 8.5, 20% v/v glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
