3PIO
Crystal structure of the synergistic antibiotic pair lankamycin and lankacidin in complex with the large ribosomal subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 170.590, 410.204, 695.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.995 - 3.247 |
| R-factor | 0.253 |
| Rwork | 0.252 |
| R-free | 0.29400 |
| Structure solution method | ISOMORPHOUS REPLACEMENT |
| Starting model (for MR) | 2zjr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.016 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.995 | 3.360 |
| High resolution limit [Å] | 3.247 | 3.247 |
| Rmerge | 0.178 | |
| Number of reflections | 353603 | |
| <I/σ(I)> | 6.1 | 1.2 |
| Completeness [%] | 93.3 | 34 |
| Redundancy | 3.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP. 15% 2:1 ethanol:methylpentanediol was diffused to a hanging drop of 180 AU/ml solution of D50S. |
